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Great work by Candice, Madeleine and Adeel reviewing and explaining how non-invasive reporter gene imaging can aid the development and clinical translation of cell-based therapies. We describe the underlying principles governing non-invasive in vivo long-term cell tracking in the preclinical and clinical settings, including available imaging technologies, reporter genes, and imaging agents as well as pitfalls related to experimental design.(https://doi.org/10.1016/j.ymthe.2020.03.016)